Choosing the right molecular instruments to equip a molecular laboratory requires consideration of various factors to ensure that the equipment meets the specific research and experimental needs of your laboratory. Here are the key aspects:
Technical specifications of PCR and real-time PCR devices that you should pay special attention to when purchasing:
Capacity and Volume: Consider the capacity and volume requirements of samples. Higher capacity devices are more expensive but are efficient for larger labs.
Accuracy and sensitivity: Ensure that the equipment has high accuracy and sensitivity, especially for real-time quantification and sequencing techniques.
Software and data analysis: Check if the software provided with the device is user-friendly and meets your data analysis needs.
Introducing the best and most efficient thermo cycler PCR device:
Veriti:
This device with high accuracy and standard and fast speed capability, reasonable price and very easy to use, fully meets your needs. The touch screen is 6.5 inches, it has capacities of 60-96-384 wells and has a gradient, and the 96-capacity model device includes six independent temperature blocks.
Introducing the best and most efficient real-time PCR devices:
Rotor gene Q :
It is considered among the most efficient and reliable devices in the field of real time. This device has 5 color filters and the device calibration system is automatic and does not need to use ROX color in the reading. There is also no need for short-term calibrations. It has 3 rotors 36-72-100 for running samples in various volumes and numbers.
step one plus :
It has high speed and accuracy with 4 optical channels. This device has 6 zones for the Plus model, which has the ability to quickly perform reactions in less than 40 minutes. Among the common features of these devices is a large touch screen and long-life LED lamps for the ROX, VIC/JOE, FAM/SYBR Green I color range. This device has the ability to perform high-resolution melt analysis. Quantitative measurement devices for protein, RNA and DNA
Nanodrop device:
Nanodrop device is a spectrophotometric device that is used to determine the concentration and purity of nucleic acids, proteins and other substances in biological solutions. This device has the following features:
• It requires a very small volume of sample (about 1 to 2 microliters) to perform the test.
• It has the ability to measure light absorption in the ultraviolet and visible range (200 to 850 nm).
• It has the ability to connect to a computer and related software and can calculate and display the results automatically.
• Ability to perform ELISA tests and classification of subgroups
• It has the ability to perform various tests such as determining the concentration of DNA, RNA, protein, carotenoid, chlorophyll, etc.
Qubit device:
The qubit device is a fluorometric device manufactured by ThermoFisher and the year of production of this device is 2017. It is used to determine the concentration and purity of nucleic acids, proteins and other substances in biological solutions. This device has the following features:
It requires a very small volume of sample (about 1 to 2 microliters) to perform the test.
It has the ability to measure fluorescence in the wavelength range of 460 to 660 nm.
It has the ability to connect to a computer and related software and can calculate and display the results automatically.
It has the ability to perform various tests such as determining the concentration of DNA, RNA, miRNA, protein, microbes, etc.
Introducing the best and most efficient sequence devices:
Sanger:
The Sanger technique is a DNA sequencing method that works based on chain termination using dideoxynucleotides. This method is known as the basis for next generation sequencing techniques, which are able to sequence larger genomes with higher speed and accuracy. To perform the Sanger technique, special devices are needed that can perform the PCR reaction and measure the fluorescence signals. These devices are generally known as DNA spectrometers (DNA sequencers) and use different technologies such as capillary electrophoresis, electronic microchips, optical microarrays, etc. Some of the most important devices used for the Sanger technique are: The ABI 3130 Genetic Analyzer is a fluorescence-based DNA analysis system with 4 parallel capillaries. This device enables high-quality data generation and sample processing for research laboratories. The ABI 3130 uses a 4-capillary array to process samples in 96- or 384-well blocks.
The Genetic Analyzer 3500 sequence device is a DNA sequencing device that uses capillary electrophoresis technology to separate and identify DNA fragments. This device can simultaneously sequence 8 or 24 samples with high speed and accuracy. This device is used to perform genetic tests, diagnosis of genetic diseases, etc. This device uses Data Collection v1.0 software to control and measure fluorescence signals. The 3500 Genetic Analyzer is available in two configurations: 3500 (8-capillary) and 3500xL (24-capillary), allowing laboratories to choose based on their needs.
The ABI 3500 sequencing machine is very important in the fields of scientific research and disease diagnosis due to its high accuracy and automatic capabilities. If you need to perform DNA sequencing analyzes with high accuracy and automation capability, ABI 3500 device is one of the right choices.
NGS:
NGS technique or new generation sequencing is an advanced method for DNA or RNA sequencing that can read and decode millions of pieces of genetic molecules simultaneously and quickly. This method is used for the study of genomics, transcriptomics, epigenetics, metabolomics, etc. and can provide useful information about the structure, function and diversity of genes, mutations, gene expression, gene regulation, etc. This method is also useful for diagnosing and treating genetic diseases, cancer, infections, etc.
To perform the NGS technique, special devices are needed that can perform sequencing reactions and measure fluorescence signals. These devices are generally known as NGS sequencers and use different technologies such as capillary electrophoresis, electronic microchips, optical microarrays, etc. Some of the most important devices used for NGS technique are: The Illumina MiSeq device uses sequencing by synthesis technology for DNA or RNA sequencing and can sequence up to 25 million pieces of DNA in each reaction.
Ion Torrent PGM is a DNA sequencing device that uses proton sequencing technology to determine the sequence of DNA or RNA. Using a semiconductor chip, this device measures the pH changes that occur during the PCR reaction and records it as a digital signal. This device can sequence up to 5 million pieces of DNA in each reaction and is used for studying genomics, transcriptomics, epigenetics, etc.
One of the key differences between these two devices:
Cost and speed of sequencing:
The Ion Torrent PGM device has a lower sequencing cost and speed than the Illumina MiSeq device. For example, to sequence a 5 Mb bacterial genome, the Ion Torrent PGM machine requires 2 hours and $100, while the Illumina MiSeq machine requires 15 hours and $200.